Figure 6. Fd-binding assay of protein complexes in the stromal fraction of spinach chloroplasts.

Stromal proteins extracted from spinach chloroplasts by using potassium phosphate buffer at pH 6 (lane 1) or pH 8 (lane 6) were mixed with Fd resin, and the resulting unbound fractions (lanes 2&7), washed fraction (lanes 3&8) and fractions eluted with first (lanes 4&9) and second (lanes 5&10) one-column volume of the same buffer containing 0.2 M NaCl were collected and analyzed by western blots after BN-PAGE.