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. 2014 Oct 1;9(10):e109307. doi: 10.1371/journal.pone.0109307

Table 1. Primers used in the studya.

Primer Sequence (5′ to 3′)
P1F AAATTCATGCCATGGACGACAACATAATAGACG
P1R TTTCCGCTCGAGTCATAAAGTGATTTCCTTGTTCTC
P2F AAATCATGAACGACAACATAATAGACGTACATTCC
P2R AAAGGATCCACCAGTATTAAGAGTAATAAGAATATAAG
P3F AAAGCTAGCAGGAAACAGCTATGACCATGATTAC
P3R TGCCAAGCTTGCATGCCTGCAG
P4F GACCTGCAGGCATGCAAGCTTGG
P4R AAAGCTAGCGTCTTGATTATCGGGCGAAGAG
P5F TAATTCCATATGAACGACAACATAATAGACG
P5R TTTCCGCTCGAGTAAAGTGATTTCCTTGTTCTC
a

The underlined sequences are restriction enzyme sites: a BamHI site (P2R), a BspHI site (P2F), a NdeI site (P5F), a NcoI site (P1F), NheI sites (P3F and P4R), PstI sites (P3R and P4F), and XhoI sites (P1R and P5R).