Table 1. Primers used in the studya.
Primer | Sequence (5′ to 3′) |
P1F | AAATTCATGCCATGGACGACAACATAATAGACG |
P1R | TTTCCGCTCGAGTCATAAAGTGATTTCCTTGTTCTC |
P2F | AAATCATGAACGACAACATAATAGACGTACATTCC |
P2R | AAAGGATCCACCAGTATTAAGAGTAATAAGAATATAAG |
P3F | AAAGCTAGCAGGAAACAGCTATGACCATGATTAC |
P3R | TGCCAAGCTTGCATGCCTGCAG |
P4F | GACCTGCAGGCATGCAAGCTTGG |
P4R | AAAGCTAGCGTCTTGATTATCGGGCGAAGAG |
P5F | TAATTCCATATGAACGACAACATAATAGACG |
P5R | TTTCCGCTCGAGTAAAGTGATTTCCTTGTTCTC |
The underlined sequences are restriction enzyme sites: a BamHI site (P2R), a BspHI site (P2F), a NdeI site (P5F), a NcoI site (P1F), NheI sites (P3F and P4R), PstI sites (P3R and P4F), and XhoI sites (P1R and P5R).