Figure 3. Resveratrol decreases cytokine–induced pro-inflammatory mediators production more efficiently than 5-ASA, in HT-29 cells.

Cells were pre-incubated with 25 µM Resv or 500 µM 5-ASA or both (25 µM Resv plus 500 µM 5-ASA) and then exposed to cytokines (Cyt) for 24 hours or 16 hours for NO and PGE₂, respectively. The NO (A) and PGE₂ (B) production in cells was measured as described in “Materials and Methods”. Values are mean ± SEM of at least three independent experiments, each one in duplicate. ^*** P<0.001 vs Control, ^## P<0.01, ^### P<0.001 vs Cytokines.