Figure 4. Resveratrol suppresses cytokine-induced iNOS and COX-2 expression more efficiently than 5-ASA, in HT-29 cells.

Cells were pre-incubated with 25 µM Resv or 500 µM 5-ASA or both (25 µM Resv plus 500 µM 5-ASA) and then challenged with a combination of cytokines. iNOS (A) and COX-2 (B) expressions were evaluated after 24 hours or 16 hours, respectively, in total extracts by Western blotting, as described in “Materials and Methods”, and expressed as percentage of cytokine-stimulated cells. Values are mean ± SEM of at least three independent experiments, each one in duplicate. ^*** P<0.001 vs Control, ^## P<0.01, ^### P<0.001 vs Cytokines and ^$$$ P<0.001 vs ASA plus Cytokines.