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. 2014 Sep 25;15(1):814. doi: 10.1186/1471-2164-15-814

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© Beer et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Validation of the miRNA data by qPCR. qPCR revealed a strong correlation between the microarray and PCR data. Expression values were normalized relative to values in non-irradiated PBMCs. The expression of miR-99b* (A), miR-887 (B), and miR-4299 (C), was significantly higher in irradiated PBMCs compared to non-irradiated PBMCs 20 hours after irradiation. Relative gene expression levels were calculated based on the mean value of four samples using the comparative Ct method. RNU44 served as internal reverence miRNA. Data are presented as the mean + SD. *p < 0.05, paired T-test; n = 4.