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. Author manuscript; available in PMC: 2014 Oct 2.
Published in final edited form as: Am J Biomed Sci. 2012;4(1):14–25. doi: 10.5099/aj120100014

Figure 4.

Figure 4

Effect of compound 3 on the activation of Akt and STAT. Colon 38 cells were treated with increasing doses (0 to 10 μg/mL) of compound 3 for 24 hr. Cells were then removed, lysed with lysis buffer. Equal amounts of lysates were subjected to Western blotting using antibodies against phospho-Akt and Akt (A), phosphor-STAT3 and STAT3 (B). Human Caco-2 cells were treated with compound 3 (0–200 μg/mL) for 24 hr and processed as described in colon 38 cells (C). Antibody against actin was used as “house-keeping protein.” Densitometric analysis was performed to measure the band intensity for each blot. Values are means ± S.D. * p < 0.05, ** p < 0.005 vs. medium control, n = 3.