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Caveolin-1 deficiency alone (without inflammatory challenge) results in increased circulating immune cells. Flow cytometry was performed on peripheral blood as described. Significant increases in total immune cells and in monocytes were observed in Cav-1 KO mice compared with WT controls (n = 10 WT and n = 11 Cav-1 KO samples, mean ± SEM, ***P < 0.001, *P < 0.05, unpaired t-test).
