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. 2014 Oct 2;5:498. doi: 10.3389/fmicb.2014.00498

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Copyright © 2014 Bailly, Laguerre, Correc, Dupont, Kurth, Pfannkuchen, Entzeroth, Probert, Vinogradov, Lechauve, Garet-Delmas, Reichert and Hartenstein.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) A net-like structure labeled by a VDAC antibody (in green) covering the epidermal surface of a juvenile connecting putative monociliated sensory receptors and gland openings. (B) The circles appearing on the magnification (focus on anterior tip = “the head”) are schematically represented in (C) (modified from Smith and Tyler, 1986: blue circles are sensory receptors, other circles represent different gland types openings). Note at the top of the anterior tip in B a dense and honeycombed-like structure corresponding to frontal gland extremity tip. The white arrows in (B) shows 3 dense and localized signals flanking the frontal gland that are ends of projections coming from a deeper cell body-like structure shown in (D). (E) A specific signal (in red) using ATPsynthase human antibody localized in the brain area in a juvenile. (F) Anterior tip of an adult showing in green (surrounding the statocyst) the specific signal of S. roscoffensis neuroglobin co-localized with brain (blue is Dapi and Red auto-fluorescence of algae). (G) A specific signal (in red) using glial cell directed human antibody (GFAP) localized in the brain area of a juvenile. (H) Adult RFaminergic nervous system. (I) Phalloïdin staining showing longitudinal, circular, and transversal muscle fibers. (Ja,Jb) Human P53 (tumor suppressor) antibody reveals dispersed and non-ordered gland-like openings on the surface.