Fig. 3.

Regulation of central metabolic enzymes by acetylation. (A) Acetylation of metabolic enzymes expressed in ΔcobB and Δpat strains. His-tagged GapA, AceA, or AceK proteins were over-expressed in the WT, ΔcobB, and Δpat strains and purified to homogeneity. Equal amounts of each protein were used and acetylation was determined. SDS PAGE indicates SDS polyacrylamide gel electrophoresis. (B and C) GapA, AceA, and AceK activities by Pat-mediated acetylation and CobB-mediated deacetylation. His-tagged GapA, AceA, and AceK proteins were purified from WT S. enterica and subjected to in vitro acetylation by Pat or deacetylation by CobB. (B) Reciprocal regulation of glycolytic and gluconeogenic activities of GapA by Pat and CobB. (C) Reciprocal regulation of AceA activity and AceK-controlled ICDH activities by Pat and CobB in vitro. Error bars indicate SD of three measurements.