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. Author manuscript; available in PMC: 2014 Oct 2.
Published in final edited form as: J Neurochem. 2006 Jun 19;98(4):1229–1241. doi: 10.1111/j.1471-4159.2006.03962.x

Table 1.

Information about the primers used for conventional and real-time PCR

Foward primer Reverse primer Amplicon size
(bp)
Tm (°C) Final
concentration
(nm)
Gapdh Glyceraldehyde-3-phosphate dehydrogenase
CAGCCTCGTCTCATAGACAAGATG CAATGTCCACTTTGTCACAAGAGAA 106 81 300
Fos FBJ osteosarcoma related oncogene
GCCAAGTGCCGGAATCG AGTTGATCTGTCTCCGCTTGGA 62 83 300
Btg2 β-cell translocation gene 2, antiproliferative
CGAGCAGAGACTCAAGGTTTTCA ATAGCCGGAGCCCTTGGA 103 81 300
Siah2 Seven in absentia 2
AAGGTCGCCTCGGCAGTT GGACGGTATTCACAGATGTCTTCA 110 81 300
Sgk Serum/glucocorticoid regulated kinase
GGGACAACGTCCACCTTCTGT CAGGCCATAGAGCATCTCATACAA 129 84 300
NFκB Nuclear factor of kappa light chain gene
enhancer in B-cells, p105
AGGATTTCGATTCCGCTACGT CCAACTGAACGATAACCTTTGCA 140 81 300
Adnp Activity-dependent neuroprotective protein
TTGGGTTGGAATACTGTAAAGAACATATA CCGATAGTCCTGATTTTTTGTAAGAGA 134 74 500
PAC1 PAC1 receptor
CCCTGACTGCTCTCCTCCTGCTGCCTAT CAGGGCAGCTCACAAGGACCATCTCACC 213 82 300
PAC1 PAC1 receptor *
CTTGTACAGAAGCTGCAGTC GGTGCTTGAAGTCCATAGTG short 281 80 1000
hop 365 81
hip-hop 449 81
VPAC1 VPAC1 receptor *
GCCCCCATCCTCCTCTCCATC TCCGCCTGCACCTCACCATTG 298 81 1000
VPAC2 VPAC2 receptor *
GTCACCTTTGCCCTCTCCATCA GCCTCTCCACCTTCTTTTCAGT 296 80 1000
*

Primers used for conventional PCR.