Table 1.
Foward primer | Reverse primer | Amplicon size (bp) |
Tm (°C) | Final concentration (nm) |
|
---|---|---|---|---|---|
Gapdh | Glyceraldehyde-3-phosphate dehydrogenase | ||||
CAGCCTCGTCTCATAGACAAGATG | CAATGTCCACTTTGTCACAAGAGAA | 106 | 81 | 300 | |
Fos | FBJ osteosarcoma related oncogene | ||||
GCCAAGTGCCGGAATCG | AGTTGATCTGTCTCCGCTTGGA | 62 | 83 | 300 | |
Btg2 | β-cell translocation gene 2, antiproliferative | ||||
CGAGCAGAGACTCAAGGTTTTCA | ATAGCCGGAGCCCTTGGA | 103 | 81 | 300 | |
Siah2 | Seven in absentia 2 | ||||
AAGGTCGCCTCGGCAGTT | GGACGGTATTCACAGATGTCTTCA | 110 | 81 | 300 | |
Sgk | Serum/glucocorticoid regulated kinase | ||||
GGGACAACGTCCACCTTCTGT | CAGGCCATAGAGCATCTCATACAA | 129 | 84 | 300 | |
NFκB |
Nuclear factor of kappa light chain gene
enhancer in B-cells, p105 |
||||
AGGATTTCGATTCCGCTACGT | CCAACTGAACGATAACCTTTGCA | 140 | 81 | 300 | |
Adnp | Activity-dependent neuroprotective protein | ||||
TTGGGTTGGAATACTGTAAAGAACATATA | CCGATAGTCCTGATTTTTTGTAAGAGA | 134 | 74 | 500 | |
PAC1 | PAC1 receptor | ||||
CCCTGACTGCTCTCCTCCTGCTGCCTAT | CAGGGCAGCTCACAAGGACCATCTCACC | 213 | 82 | 300 | |
PAC1 | PAC1 receptor * | ||||
CTTGTACAGAAGCTGCAGTC | GGTGCTTGAAGTCCATAGTG | short 281 | 80 | 1000 | |
hop 365 | 81 | ||||
hip-hop 449 | 81 | ||||
VPAC1 | VPAC1 receptor * | ||||
GCCCCCATCCTCCTCTCCATC | TCCGCCTGCACCTCACCATTG | 298 | 81 | 1000 | |
VPAC2 | VPAC2 receptor * | ||||
GTCACCTTTGCCCTCTCCATCA | GCCTCTCCACCTTCTTTTCAGT | 296 | 80 | 1000 |
Primers used for conventional PCR.