Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1995 May 23;92(11):4987–4991. doi: 10.1073/pnas.92.11.4987

Isolation and characterization of a MAGE gene family in the Xp21.3 region.

F Muscatelli 1, A P Walker 1, E De Plaen 1, A N Stafford 1, A P Monaco 1
PMCID: PMC41832  PMID: 7761436

Abstract

A human gene with strong homology to the MAGE gene family located in Xq27-qter has been isolated by using exon-trapping of cosmids in the Xp21.3 region. We have mapped and sequenced cDNA and genomic clones corresponding to this gene, MAGE-Xp, and shown that the last exon contains the open reading frame and is present in a minimum of five copies in a 30-kb interval. MAGE-Xp is expressed only in testis and, unlike the Xq27-qter MAGE genes, it is not expressed in any of 12 different tumor tissues tested. However, the gene and predicted protein structure are conserved, suggesting a similar function. MAGE-Xp is located in the 160-kb critical interval defined for the locus involved in sex determination within Xp21 and is 50 kb distal to the DAX-1 gene, which is responsible for X-chromosome-linked adrenal hypoplasia congenita.

Full text

PDF
4987

Images in this article

4988Fig. 2
on p.4988
4991Fig. 5
on p.4991

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aizawa T., Maruyama K., Kondo H., Yoshikawa K. Expression of necdin, an embryonal carcinoma-derived nuclear protein, in developing mouse brain. Brain Res Dev Brain Res. 1992 Aug 21;68(2):265–274. doi: 10.1016/0165-3806(92)90069-9. [DOI] [PubMed] [Google Scholar]
  2. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. Basic local alignment search tool. J Mol Biol. 1990 Oct 5;215(3):403–410. doi: 10.1016/S0022-2836(05)80360-2. [DOI] [PubMed] [Google Scholar]
  3. Bardoni B., Zanaria E., Guioli S., Floridia G., Worley K. C., Tonini G., Ferrante E., Chiumello G., McCabe E. R., Fraccaro M. A dosage sensitive locus at chromosome Xp21 is involved in male to female sex reversal. Nat Genet. 1994 Aug;7(4):497–501. doi: 10.1038/ng0894-497. [DOI] [PubMed] [Google Scholar]
  4. Boon T., De Plaen E., Lurquin C., Van den Eynde B., van der Bruggen P., Traversari C., Amar-Costesec A., Van Pel A. Identification of tumour rejection antigens recognized by T lymphocytes. Cancer Surv. 1992;13:23–37. [PubMed] [Google Scholar]
  5. Brasseur F., Marchand M., Vanwijck R., Hérin M., Lethé B., Chomez P., Boon T. Human gene MAGE-1, which codes for a tumor-rejection antigen, is expressed by some breast tumors. Int J Cancer. 1992 Nov 11;52(5):839–841. doi: 10.1002/ijc.2910520528. [DOI] [PubMed] [Google Scholar]
  6. Buckler A. J., Chang D. D., Graw S. L., Brook J. D., Haber D. A., Sharp P. A., Housman D. E. Exon amplification: a strategy to isolate mammalian genes based on RNA splicing. Proc Natl Acad Sci U S A. 1991 May 1;88(9):4005–4009. doi: 10.1073/pnas.88.9.4005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Celis E., Tsai V., Crimi C., DeMars R., Wentworth P. A., Chesnut R. W., Grey H. M., Sette A., Serra H. M. Induction of anti-tumor cytotoxic T lymphocytes in normal humans using primary cultures and synthetic peptide epitopes. Proc Natl Acad Sci U S A. 1994 Mar 15;91(6):2105–2109. doi: 10.1073/pnas.91.6.2105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. De Plaen E., Arden K., Traversari C., Gaforio J. J., Szikora J. P., De Smet C., Brasseur F., van der Bruggen P., Lethé B., Lurquin C. Structure, chromosomal localization, and expression of 12 genes of the MAGE family. Immunogenetics. 1994;40(5):360–369. doi: 10.1007/BF01246677. [DOI] [PubMed] [Google Scholar]
  9. De Smet C., Lurquin C., van der Bruggen P., De Plaen E., Brasseur F., Boon T. Sequence and expression pattern of the human MAGE2 gene. Immunogenetics. 1994;39(2):121–129. doi: 10.1007/BF00188615. [DOI] [PubMed] [Google Scholar]
  10. Ding M., Beck R. J., Keller C. J., Fenton R. G. Cloning and analysis of MAGE-1-related genes. Biochem Biophys Res Commun. 1994 Jul 15;202(1):549–555. doi: 10.1006/bbrc.1994.1963. [DOI] [PubMed] [Google Scholar]
  11. Gaboriaud C., Bissery V., Benchetrit T., Mornon J. P. Hydrophobic cluster analysis: an efficient new way to compare and analyse amino acid sequences. FEBS Lett. 1987 Nov 16;224(1):149–155. doi: 10.1016/0014-5793(87)80439-8. [DOI] [PubMed] [Google Scholar]
  12. Gaugler B., Van den Eynde B., van der Bruggen P., Romero P., Gaforio J. J., De Plaen E., Lethé B., Brasseur F., Boon T. Human gene MAGE-3 codes for an antigen recognized on a melanoma by autologous cytolytic T lymphocytes. J Exp Med. 1994 Mar 1;179(3):921–930. doi: 10.1084/jem.179.3.921. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kozak M. An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs. Nucleic Acids Res. 1987 Oct 26;15(20):8125–8148. doi: 10.1093/nar/15.20.8125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Larin Z., Monaco A. P., Lehrach H. Yeast artificial chromosome libraries containing large inserts from mouse and human DNA. Proc Natl Acad Sci U S A. 1991 May 15;88(10):4123–4127. doi: 10.1073/pnas.88.10.4123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Maruyama K., Usami M., Aizawa T., Yoshikawa K. A novel brain-specific mRNA encoding nuclear protein (necdin) expressed in neurally differentiated embryonal carcinoma cells. Biochem Biophys Res Commun. 1991 Jul 15;178(1):291–296. doi: 10.1016/0006-291x(91)91812-q. [DOI] [PubMed] [Google Scholar]
  16. Muscatelli F., Strom T. M., Walker A. P., Zanaria E., Récan D., Meindl A., Bardoni B., Guioli S., Zehetner G., Rabl W. Mutations in the DAX-1 gene give rise to both X-linked adrenal hypoplasia congenita and hypogonadotropic hypogonadism. Nature. 1994 Dec 15;372(6507):672–676. doi: 10.1038/372672a0. [DOI] [PubMed] [Google Scholar]
  17. Oaks M. K., Hanson J. P., Jr, O'Malley D. P. Molecular cytogenetic mapping of the human melanoma antigen (MAGE) gene family to chromosome region Xq27-qter: implications for MAGE immunotherapy. Cancer Res. 1994 Apr 1;54(7):1627–1629. [PubMed] [Google Scholar]
  18. Traversari C., van der Bruggen P., Luescher I. F., Lurquin C., Chomez P., Van Pel A., De Plaen E., Amar-Costesec A., Boon T. A nonapeptide encoded by human gene MAGE-1 is recognized on HLA-A1 by cytolytic T lymphocytes directed against tumor antigen MZ2-E. J Exp Med. 1992 Nov 1;176(5):1453–1457. doi: 10.1084/jem.176.5.1453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Walker A. P., Chelly J., Love D. R., Brush Y. I., Récan D., Chaussain J. L., Oley C. A., Connor J. M., Yates J., Price D. A. A YAC contig in Xp21 containing the adrenal hypoplasia congenita and glycerol kinase deficiency genes. Hum Mol Genet. 1992 Nov;1(8):579–585. doi: 10.1093/hmg/1.8.579. [DOI] [PubMed] [Google Scholar]
  20. Walker A. P., Muscatelli F., Monaco A. P. Isolation of the human Xp21 glycerol kinase gene by positional cloning. Hum Mol Genet. 1993 Feb;2(2):107–114. doi: 10.1093/hmg/2.2.107. [DOI] [PubMed] [Google Scholar]
  21. Weynants P., Lethé B., Brasseur F., Marchand M., Boon T. Expression of mage genes by non-small-cell lung carcinomas. Int J Cancer. 1994 Mar 15;56(6):826–829. doi: 10.1002/ijc.2910560612. [DOI] [PubMed] [Google Scholar]
  22. Worley K. C., Ellison K. A., Zhang Y. H., Wang D. F., Mason J., Roth E. J., Adams V., Fogt D. D., Zhu X. M., Towbin J. A. Yeast artificial chromosome cloning in the glycerol kinase and adrenal hypoplasia congenita region of Xp21. Genomics. 1993 May;16(2):407–416. doi: 10.1006/geno.1993.1204. [DOI] [PubMed] [Google Scholar]
  23. Zanaria E., Muscatelli F., Bardoni B., Strom T. M., Guioli S., Guo W., Lalli E., Moser C., Walker A. P., McCabe E. R. An unusual member of the nuclear hormone receptor superfamily responsible for X-linked adrenal hypoplasia congenita. Nature. 1994 Dec 15;372(6507):635–641. doi: 10.1038/372635a0. [DOI] [PubMed] [Google Scholar]
  24. van der Bruggen P., Traversari C., Chomez P., Lurquin C., De Plaen E., Van den Eynde B., Knuth A., Boon T. A gene encoding an antigen recognized by cytolytic T lymphocytes on a human melanoma. Science. 1991 Dec 13;254(5038):1643–1647. doi: 10.1126/science.1840703. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES