Fig. 4.
Survival of cerebellar granule cells cultured with neurotoxic agents. (A) Diagram of the protocol used for the culture and treatment with ethanol, H2O2 or PACAP; (B) effect of graded concentrations of ethanol on granule neurons from wild type and PACAP−/− animals; (C) effect of graded concentrations of H2O2 on granule neurons from wild type and PACAP−/− animals. Each value represents the mean (±S.E.M.) of three independent experiments performed in quadruplicate. *P< 0.05 vs. wild type; #P < 0.05 vs. no PACAP.