Table 1.

Effect of neurotrophic factors on cultured cerebellar granule cells from wild type and PACAP-deficient mice

Treatment	Wild type	PACAP-deficient
PACAP	140 ± 7**	131 ± 6#
Potassium	197 ± 12**	169 ± 10###
dbcAMP	129 ± 4**	119 ± 4#

After culturing the cells for 3–4 days with serum and 25 mM potassium, serum was removed and potassium was shifted to 5 mM. After 12 h of culture in serum free medium and 5 mM potassium, cells were treated with control medium (serum free and 5 mM potassium), PACAP (10^–7M), potassium (25 mM) or dbcAMP (10^–3 M). Cell survival was measured 24 h after treatment and reported as percentage ofcontrol. Each value represents the mean (±S.E.M.) of three independent experiments performed in quadruplicate.

^**P < 0.01 vs. wild type control.

^#P < 0.05 vs. PACAP-deficient control.

^###P < 0.001 vs. PACAP-deficient control.