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. Author manuscript; available in PMC: 2014 Oct 2.
Published in final edited form as: Peptides. 2005 Jul 11;26(12):2518–2524. doi: 10.1016/j.peptides.2005.05.015

Table 3.

Quantification of relative PACAP mRNA expression in mouse cerebellum at post-natal days 6 and 12

Post-natal day CT PACAP CT GAPDH % adult HT
6 29.23 ± 0.29 20.42 ± 0.03 11.66
12 29.54 ± 0.05 19.83 ± 0.04 6.25

PACAP mRNA was measured in total cerebellum from post-natal days 6 and 12 wild type and PACAP-deficient mice as described in Section 2. The threshold cycle number (CT) ± S.E.M. for PACAP and GAPDH transcripts was measured (n = 3), and used to calculate the concentration of PACAP mRNA in developing cerebellum as a function of its concentration in a PACAP-rich mouse reference tissue, the hypothalamus (HT).