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A, breast cancer cell lines express endogenous LIMD2. B, ectopic expression of LIMD2 in MCF-7 cells. C, siRNA-mediated LIMD2 knockdown in MDA-MB-231 cells (the Si2 and Si5 are 2 independent cell clones). Migration potentials of each cell line. Data are presented as mean ± SD. T test was performed and P value is indicated. D, morphology of the cells. Bar, 100 μm. E, soft agar colony formation assays of each cell line.
