Total locomotor reactivity to four intermittent nicotine (0.35 mg/kg, s.c.) or saline (1.0 ml/kg, s.c.) training injections (A), total locomotor reactivity to a low dose nicotine (0.1 mg/kg, s.c.) challenge (B), and percent time spent interacting with the resident rat in the SI test (C) are shown. Group means ±SEMs are plotted in line (A) and bar (B and C) graphs. In panel A, (*) represents significant effects in total locomotor reactivity to nicotine in HRs compared to saline-injected controls, whereas (#) represents significant effects in nicotine-injected LRs compared to saline-injected controls. Significance is set at p ≤ 0.005.