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Lipid-anchored myoVas diffuse freely on DOPC membranes. (A) Cartoon illustrating experimental setup of planar supported lipid bilayers. (B) Mean-squared displacement (MSD) analysis and sample trajectory (Inset) of Qdot-labeled myoVa molecule diffusing in a DOPC membrane with an apparent diffusion coefficient, D, shown. However, motors tethered to a bilayer of gel-state DPPC (C) show minimal detectable movement.
