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. 2014 Sep 11;111(38):13948–13953. doi: 10.1073/pnas.1400821111

Fig. 3.

Fig. 3.

Specificity of SBP–M13–SWNTs for OVCAR8 tumor nodules in the abdominal cavity. (A) Photographs and NIR2 fluorescence (10–50-ms exposure) of tumor nodules implanted on several peritoneal organs. (B) Quantification of nodule and organ-specific background for nodules present on the liver, intestine, and spleen (n = 8–11 nodules per organ; **P < 0.01; ****P < 0.0001; two-tailed t tests). (C) Tumor-to-intestine ratio for targeted and untargeted M13–SWNT probes. Intestinal tissue used for background intensity (+SBP, n = 6; –SBP, n = 13; **P < 0.01; two-tailed t test). (D) Representative NIR2 fluorescence and H&E staining of a positive nodule revealing characteristic tumor histology. (E) Immunofluorescence staining reveals colocalization of M13–SBP–SWNTs conjugated to AlexaFluor750 dye with SPARC expression in an NIR2-positive nodule. [Scale bars, 10 mm (A), 10 mm (D, NIR2), 250 μm (D, H&E liver nodule), 125 μm (D, H&E nodules), 5 mm (E, NIR2), and 2.5 mm (E, SPARC, AF750–M13).]