Figure 5.

ELISA analysis of PEDF secretion by hES-RPE (passage 3) derived from hES-3 embryonic stem cell line and control cells. All PEDF values in the supernatants were normalized to total protein levels of the corresponding cellular lysates. Numerical values of PEDF concentration are shown in the box at the top of each bar. The polarized hES-RPE and the polarized fRPE secreted approximately 100-fold greater levels of PEDF than the nonpolarized hES-RPE, fRPE, and ARPE19 cells. ELISA revealed that polarized hES-RPE secreted twice as much PEDF as that of polarized fRPE. Results represent three independent experiments. *P < 0.05; **P < 0.01.