Figure 6.

Confocal images of PEDF immunofluorescence in polarized (A1–I1) and nonpolarized (A2–I2) hES-RPE (passage 3) taken from equivalent levels in the apical region (A–C), middle region (D–F), and basal region (G–I). The majority of PEDF is localized to the apical cytoplasm of polarized hES-RPE cells (A1–I1), whereas PEDF was reduced in amount and found more in the central region in the cytoplasm of nonpolarized hES-RPE cells (A2–I2). Immunoreactivity for PEDF was detected using secondary antibodies conjugated to FITC (green). Nuclei are counterstained with DAPI (blue). Merged images show both PEDF immunoreactivity and DAPI nuclear staining. Images are representative of three independent experiments.