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. 2011 Mar 21;52(3):1573–1585. doi: 10.1167/iovs.10-6413

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Figure 8. — BrdU incorporation analysis of RPC proliferation after culture in various CM. The RPCs cultured in the polarized hES-RPE (passage 3) CM, polarized fRPE CM, or RPC medium with 50 ng/mL PEDF supplement had more proliferating cells (BrdU-positive cells) than those cultured in the regular RPC medium, HEK-CM, polarized fRPE CM, or hES-RPE CM, with anti-PEDF antibody. (A–H) Light microscopy images of BrdU-positive RPCs. (A) RPCs cultured in the regular RPC medium; (B) RPCs in HEK-CM; (C) RPCs in RPC medium with 50 ng/mL PEDF; (D) RPCs in RPC medium with 50 ng/mL PEDF and 20 μg/mL anti-PEDF antibody; (E) RPCs in the polarized fRPE CM; (F) RPCs in the polarized fRPE CM with 20 μg /mL anti-PEDF antibody; (G) RPCs in the polarized hES-RPE CM; (H) RPCs in the polarized hES-RPE CM with 20 μg/mL anti-PEDF antibody. (I) The quantification of BrdU-positive RPCs from three individual experiments. The RPCs cultured in the polarized hES-RPE CM, polarized fRPE CM, or RPC medium with recombinant PEDF supplement had a significantly higher number of BrdU-positive cells than those cultured in the regular RPC medium, HEK-CM, polarized fRPE- CM or hES-RPE CM, and recombinant PEDF with anti-PEDF antibody. **P < 0.01.