Table 1. Mutations beneficial to ID8 under two selection regimes.

Label	Selection		Protein function	Protein name	Aa position	Aa	Nuc position	Nuc
T1	80°C	1	coat	F	5	T A	2578	A G
T2	80°C	1	coat	F	355	P S	3628	C T
T3	80°C	1	spike	G	168	R C	4484	C T
T4	80°C	2	spike	G	38	R C	4094	C T
P1	pH 1.5	2	coat	F	77	I T	2792	T C
P2	pH 1.5	1	spike	G	65	T A	4175	A G
P3	pH 1.5	2	pilot	H	71	A V	4738	C T

The seven beneficial mutations identified in this study affected three different viral structural proteins: the major coat protein F, the spike protein G, and the pilot protein H. Positions are based on the published genome sequence of ID8 (GenBank accession # DQ079898). Nuc, nucleotide; n, number of lineages with mutation; Nuc, nucleotide change; Aa, amino acid; Aa, amino-acid change.