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. 2014 Oct 2;10(10):e1004654. doi: 10.1371/journal.pgen.1004654

Figure 2. Loss of Polq contributes to chromosomal instability both spontaneously and in the presence of DNA damage.

Figure 2

Polq+/+ or Polq−/− bone marrow stromal cells plated in chamber slides were exposed to (A) X-rays or (B) etoposide. 48 hr after damage cells were fixed and stained with DAPI to enumerate cells with micronuclei. Counts represent the average percentage of cells with micronuclei scored in three independent experiments. (Slopes for X-ray and etoposide-induced MN: Polq+/+ clone 1 (2.8, 0.73); Polq+/+ clone 2 (3.1, 0.85); Polq−/− clone 1 (4.8, 1.2); Polq−/− clone 3 (6.2, 1.3). The frequency of spontaneous micronuclei for each of the clones in Figure 2A and 2B were combined to generate (C) total spontaneous micronuclei observed for all genotypes. The p-value was determined by Wilcoxon Mann Whitney rank sum test. Polq+/+ or Polq−/− (D) bone marrow stromal cells and (F) mouse embryonic fibroblasts were plated in growth medium in triplicate. Cells were counted at the indicated days and cumulative population doublings were recorded. The experiment was repeated three times. (E and G) Absolute quantification of Polq transcript numbers in three independent experiments.