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. 2014 Oct 2;10(10):e1004654. doi: 10.1371/journal.pgen.1004654

Figure 4. Insertions >1 nt at CSR junctions are Polq-dependent.

Figure 4

Isolated wild-type (WT) and Polq−/− (KO) naïve splenic B cells were stimulated for CSR and either mock-treated or treated with NU7026. (A) Cells were assayed for IgG1 levels (y-axis) by flow cytometry. The x-axis sort is on forward scatter (FSC) for cell viability. Aicda−/− splenic B cells were used as a negative control. Numbers in boxes show the percentage of the population that is IgG1 positive; in (B) these data are plotted relative to wild-type. Genomic DNA isolated from B cells of wild-type (C) and Polq KO (D) mice was amplified by PCR and 100 Sμ-Sγ1 junctions from each group were sequenced and analyzed for overlaps and insertions at breakpoints. (E) Insertions >1 nt are plotted; p-values were determined by a two-tailed Fisher's exact test. Cell viabilities were comparable between genotypes.