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. 2014 Oct 2;9(10):e108276. doi: 10.1371/journal.pone.0108276

Figure 3. JAK2/STAT3 is involved in the loud sound induced up-regulation of pro-inflammatory transcript levels.

Figure 3

Mice were treated with the JAK2/STAT3 pathway inhibitor JSI-124 (Cucurbitacin I) (1 mg/kg) or DMSO (Control) at 48 hours, 24 hours and 1 hour prior to exposure to 110 dB SPL 4–48 kHz for 3 hrs. (A) Representative Western blots of total cochlea extracts for levels of pSTAT3 (Y705) and total STAT3 at 4 hours post noise exposure (NE) in four different treatment groups: Control, JSI-124, JSI-124+NE, and NE animals. (B) Fold change in pSTAT3 levels in the different treatment groups relative to Control. The noise-induced increase of pSTAT3 levels was attenuated by prior treatment with JSI-124. (C) Quantitative real-time PCR was used to assess IL-6, MCP1, and NOX3 transcript levels in total RNA extracted from whole cochlea from the four different treatment groups at 4 hours post NE. With this NE protocol, increases in all three transcript levels were observed that was attenuated by prior JSI-124 treatment. Data presented as mean+SEM, n = 3 mice/group. *P<0.05; **P<0.001.