Figure 2. SLA induced IFNγ secretion in WB of active VL patients is not dependent on plasma proteins or RBC.

IFNγ in SLA stimulated WB from active VL patients (n = 8/9) following replacement of plasma or lysis or RBC as indicated (WB = whole blood untouched; HI-Auto = replacement with heat inactivated [12] autologous plasma; HI-FCS = replacement with HI-FCS). Net responses (stimulated minus unstimulated)+SD are shown. The stimulation indices (stimulated/unstimulated) are indicated above each bar to as an additional comparison. Comparisons between treatments were made using Wilcoxon matched paired test; there were no significant differences between the different culture conditions.