Skip to main content
. 2014 Oct 2;8(10):e3198. doi: 10.1371/journal.pntd.0003198

Figure 3. CD4 cells are necessary for and the source of IFNγ in SLA stimulated WB from patients with active VL.

Figure 3

IFNγ production in SLA stimulated WB cultures of active VL patients following depletion of a) CD4 and CD8 positive cells. b) Effect of pan-HLA-DR blockade on SLA induced IFNγ, compared to IgG2a isotype control treatment c) Intra cellular IFNγ lymphocytes, following SLA stimulation of WB culture. The dot-plots to the left (red) show IFNγ production in total gated lymphocytes and the dot-plots to the right (purple) show SLA induced IFNγ in gated CD3+ cells. In the graph the frequencies of IFNγ+ CD4+ T cells are shown; combined results of three different experiments. d) Correlation between percentages of IFNγ positive cells in gated CD3+ lymphocytes as determined by ICS and IFNγ levels in WB supernatants following 24 hours of SLA stimulation. e) IL-10 levels in SLA stimulated WB supernatants following removal of CD4 and CD8 positive cells. Plasma was replaced with HI-FCS prior to incubation with MicroBeads or HLA-DR blockade, MB = mock treatment with magnetic beads directed against FITC. Each dot represents one sample. Net SLA response (e.g. stimulated minus unstimulated) samples are shown, if not otherwise indicated. Comparison between treatments was made using Wilcoxon matched paired test and statistical significances are indicated with *** p<0.001, ** p<0.01 and * p<0.05.