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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: J Pediatr Surg. 2013 Oct 5;49(1):159–165. doi: 10.1016/j.jpedsurg.2013.09.051

Figure 4. Inhibition of AURKA reduced angiogenesis in vitro and decreased VEGF mRNA levels and secretion.

Figure 4

(A) HUVECs cultured in cell culture supernatant from BE(2)-C/shAURKA cells resulted in decreased tubule formation when compared to cells grown in supernatant from BE(2)-C/shCON cells. (B) HUVECs cultured in supernatant from BE(2)-C cells treated with MLN8237 resulted in decreased tubule formation when compared to cells grown in supernatant from BE(2)-C cells treated with vehicle alone. (C) BE(2)-C/shCON and BE(2)-C/shAURKA cells were seeded in a 6-well plate, then serum-starved for 48 h. Cell culture supernatant was then collected and VEGF ELISA was performed. (D) AURKA silencing decreased mRNA levels of VEGF by ∼6 fold as assessed by real Time-PCR. GAPDH was used as an internal control. (mean ± SEM; *=p<0.05 vs. control).