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. 2014 Oct 2;8(10):e3122. doi: 10.1371/journal.pntd.0003122

Table 1. Primers used in RT-PCR and DNA sequencing for Japanese encephalitis virus (JEV).

Primer name Sequence 5′-3′ Primer used
JE-5UTRF AGA AGT TTA TCT GTG TGA ACT TCT TGG PCR, sequencing
JE-616R CCT CAC ACA TGT AGC CGA CGT CT Sequencing
JE-747R TTC GCT TGG AAT GCC TGG TCC G Sequencing
JE-747F CGG ACC AGG CAT TCC AAG CGA A Sequencing
JE-1448R GGA AGC ATT GAC ACA TGT GCA AAA TT PCR, sequencing
JE-1309F AGA ACA ATC CAG CCA GAA AAC ATC PCR, sequencing
JE-1360F CGC TGA ATA ATT CCC ATG GTT TTC Sequencing
JE-1839F AGG CTG AAA ATG GAC AAA CTG GC Sequencing
JE-1878R GGT TGT GCC TTT CAG AGC CAG TTT Sequencing
JE-2515R ATC TCT TTT CTT GTG ATG TCA ATG GC Sequencing
JE-2602R AGG GAT CTG GGC GTT TCT GG Sequencing
JE-2636R GCC TTC CTT GTG CGC TTT GT PCR, sequencing
JE-2340F GGG AAT GTC TTG GAT CAC ACA AGG PCR, sequencing
JE-2926F TGG AAC AGC ATG CAA ATC GAA GA Sequencing
JE-3032R CCT ATG ATC GCT CCA TCA CAC TC Sequencing
JE-3630R GGT ACG GAA TGG AAA TCA GAC CTG PCR, sequencing
JE-3467F CAA TCT GGC CGT CCA CCT CTT GC PCR, sequencing
JE-4169F CCA CTA TAG CTG CCG GAC TAA TGG Sequencing
JE-4324R CTG CCA GCA TGA AGG GTA TTG AC Sequencing
JE-4946R TGG CAC ACA ACT AGA GGA GCA GC PCR, sequencing
JE-4756F GGT TTT GTC TGG ATG TTT ACT GC PCR, sequencing
JE-4946F GCA GTA AAC ATC CAG ACA AAA CC Sequencing
JE-5424R TGA CAT CAG TCT ATG GGT CAG AGT Sequencing
JE-5424F ACT CTG ACC CAT AGA CTG ATG TCA Sequencing
JE-6096R CCA TCC CCC ATA ACC AGT GCA AG PCR, sequencing
JE-5946F TAA CAT GAT CTT TGC CTC TGT CC PCR, sequencing
JE-6096F GGA CAG AGG CAA AGA TCA TGT TA Sequencing
JE-6678F GAT GCA GCG AAA GGG TAT AGG GAA Sequencing
JE-6770R GTT CCA GGA ACC TCT GCC GCC CA Sequencing
JE-7388R TGG ATG GCA AGC AGA AGC ACT CAG PCR, sequencing
JE-7293F ACA TCA GTG GCG ACC ATT CCG TC PCR, sequencing
JE-7469F CAT AGG GGT AAG CGT GGC AGC GTT Sequencing
JE-7829F GAG GAC ATC CGG TTT CGC GAG Sequencing
JE-8030R CTC TGC ATG AGC ATC GGT TCT TC Sequencing
JE-8579R GCG AAT GGA TCG CAC AGT GTG GAG PCR, sequencing
JE-8403F GGA TGC TCA GGG TCT TTG TGC CA PCR, sequencing
JE-8579F TGG CAC AAA GAC CCT GAG CAT CC Sequencing
JE-9185R GAA CAG AAT CAA TGG AGC ACA GC Sequencing
JE-8926F TCT CGG CTC AGC CAA TGG TCT TC PCR, sequencing
JE-9185F GAA GAC CAT TGG CTG AGC CGA GA PCR, sequencing
JE-9719R TCA AGA GAA GAC CAA AGG GGG AGT Sequencing
JE-10266R ATG GCG ATC AGC GGA GAC GAC T PCR, sequencing
JE-9489F CTG TGT CGT CAA GCC GCT GG PCR, sequencing
JE-9664F GTG GCG AAT CTG TCG TCC AGC GG Sequencing
JE-9703F GTG GTC ATC CAC TCT CCT TTC GAG Sequencing
JE-10086R CCA GAT GTC CTC ACG CTT TCC CAC Sequencing
JE-10230R GGT TGC TCT GGA TCG CGT TCC GAT Sequencing
JE-10266F ATC GGA ACG CGA TCC AGA GCA ACC Sequencing
JE-10488F ACT GGG TAG ACG GTG CTG CCT G Sequencing
JE-10980R AGA TCC TGT GTT CTT CCT CAC CAC PCR, sequencing