Figure 3. ATMT of P. brasiliensis to certify the Zeocin/ZeoR positive selection system and the terminal GP43 gene transcription region.

(A). Four independent transformation experiments were performed by co-cultivating 1×10⁶ viable Pb18 yeast cells with A. tumefaciens at different ratios. A. tumefaciens carried the “Prm_CBP1::Shble::Ttr_GP43” cassette to express the ZeoR selection marker (*, P = 0.0002; **, P = 0.001). (B). Three independent transformation experiments were performed by co-cultivating 1×10⁶ viable Pb18 yeast cells with A. tumefaciens at a ratio of 1∶100 and recovering the yeast cells using two different selective media (*, P = 0.02).