Figure 5. Epifluorescence microscopy analysis of yeast cells expressing the bifunctional selective marker.

Yeast cells carrying integrated cassettes under the transcriptional regulation of Prm_CBP1 (A), Prm_Act (B) or Prm_GP43 (C) were collected from late-log phase cultures and visualized through confocal microscopy. (D). Yeast cells carrying the integrated “Prm_CBP1::Shble::Ttr_GP43” expression cassette was used as negative control. Scale bar: 10 µm.