Figure 2. RuBzNO₂ is highly effective in killing trypomastigotes and does not show cytotoxicity.

Trypomastigote forms of T. cruzi (A) or spleen cells of mice (B) were cultured with serial dilutions of RuBzNO₂ or Bz for 24 h at 37°C. A. Live parasites were counted by microscopy to determine the trypanocidal activity of the compounds. B. Dead cells were stained with propidium iodide and quantified by flow cytometry. The bars indicate means+SEM of duplicates and are representative of three independent experiments.