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. 2014 Oct 2;8(10):e3211. doi: 10.1371/journal.pntd.0003211

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© 2014 Castro-Sesquen et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Poly(NIPAm/trypan blue) particles capture and concentrate trypsin in spiked normal urine samples. P: Particles (trypsin: 3 ng/ml); Out: Outside the particles (unbound proteins); In: Inside the particles (bound proteins)); IS: Initial Solution. B. H49 and 1F8 T. cruzi antigens spiked in normal urine samples are protected from trypsin digestion by poly(NIPAm/trypan blue) particles. −P: T. cruzi antigen and trypsin without particles. + P: T. cruzi antigen and trypsin with particles. IS: Initial Solution of T. cruzi antigen without trypsin.