Skip to main content
. 2014 Oct 2;9(10):e108868. doi: 10.1371/journal.pone.0108868

Figure 2. Growth-phase dependent nos (A) and pdt (B) expression.

Figure 2

Total RNA was isolated at the indicated time points from n = 3 biological replicates of UAMS-1 grown under highly aerated (light grey bars; 250 RPM, 1∶10 volume-to-flask ratio) or low-oxygen (dark grey bars; 0 RPM, 7∶10 volume-to-flask ratio) conditions. Quantitative real-time PCR was performed on reverse-transcribed cDNA from each sample using nos (A) or pdt (B) specific primers. The Livak (2-ΔΔCt) method was used to determine relative fold change of nos and pdt expression, using measured sigA expression as the reference gene and the 2 hour aerobic sample as the calibrator. Error bars  =  standard error of the mean (SEM). *denotes statistical significance between aerobic and low-oxygen fold-change values at each indicated time point (Two-Tailed T-Test, p<0.01); •denotes statistical significance relative to t = 2 hour low-oxygen time point (Dunnett's Test, p<0.05).