Table 1. Bacterial strains and plasmid constructs used in this study.
| Strain or plasmid name | Description | Reference or source |
| Escherichia coli | ||
| DH5α | Host strain for construction of recombinant plasmids | [68] |
| Staphylococcus aureus | ||
| RN4220 | Easily transformable restriction deficient strain | [69] |
| UAMS-1 | Osteomyelitis clinical isolate | [70] |
| KR1010 | UAMS-1 nos::erm insertion mutant | This work |
| KR1013 | UAMS-1 Δpdt deletion mutant | This work |
| Plasmids | ||
| pTR27 | nos::erm allele-replacement plasmid; ErmR/CmR | This work |
| pKR13 | Δpdt allele-replacement plasmid; CmR | This work |
| pMKnos | nos complementation plasmid; CmR/AmpR | This work |
| pMKpdt | pdt complementation plasmid; CmR/AmpR | This work |
| pCRBlunt | E. coli cloning plasmid; KmR | Invitrogen |
| pCR2.1 | E. coli cloning plasmid; KmR/AmpR | Invitrogen |
| pBSK | E. coli cloning plasmid; AmpR | Stratagene |
| pBT2 | Temperature-sensitive shuttle vector; CmR/AmpR | [25] |
| pMK4 | Shuttle vector; CmR/AmpR | [29] |