Table 2. Measurement of ventricular dimensions in WT and GPR55−/− mice.
Young Mice (10 week old) | Mature Mice (8 month old) | |||
WT (n = 15) | GPR55−/− (n = 15) | WT (n = 14) | GPR55−/− (n = 14) | |
HW∶BW (mg/g) | 3.07±0.06 | 3.19±0.1 | 3.98±0.06* | 3.59±0.02† |
LV Wall Thickness (mm) | 1.68±0.04 | 1.79±0.11 | 1.92±0.05* | 1.65±0.07† |
RV Wall Thickness (mm) | 0.39±0.02 | 0.38±0.04 | 0.36±0.04 | 0.40±0.01 |
Interventricular Septal Thickness (mm) | 1.25±0.04 | 1.22±0.06 | 1.31±0.01 | 1.35±0.02 |
Chamber Area (% of LV) | 13.9±0.9 | 15.5±1.3 | 12.8±0.7 | 13.2±0.8 |
Cardiomyocyte CSA (µm2) | 89±9 | 94±8 | 145±5* | 144±7 |
Nuclei number (nuclei/mm2) | 6726±284 | 6456±169 | 6471±393 | 4983±405# † |
Ventricular dimensions did not differ significantly between young WT and GPR55−/− mice, however mature GPR55−/− mice were characterised by significant myocardial remodelling; including a reduction in left ventricular (LV) free wall thickness, myocardial nuclei number, and HW∶BW, and increased collagen deposition. Data is expressed as mean±s.e.m. (n = 14–15).
*P<0.05 vs. WT (Young);
P<0.05 vs. GPR55−/− (Young);
P<0.05 vs. WT (Mature).