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. 2014 Oct 2;9(10):e109075. doi: 10.1371/journal.pone.0109075

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© 2014 Deifl et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Pollen grains were incubated in H₂O (a), simulated lung fluid (b), 0.9% NaCl (c) and PBS (d). Supernatants taken at indicated time points were checked for their content of bGST (closed squares) and Bet v 1 (open circles) by ELISA. (B) Immunoblotting of supernatants collected after 60 min. bBP, boiled BP, SLF, simulated lung fluid.