Figure 6. Increased seeding density failed to rescue OCG defect in Adseverin knockdown monocytes.

WT, Luc KD and Ads KD (Clone F) monocytes were seeded at three initial plating densities in 12-well tissue culture plates, low (1×10⁴ cells/well), normal (5×10⁴ cells/well) and high (1×10⁵ cells/well). Cells were stimulated for 4 days with sRANKL to stimulate OCG. Representative TRAcP stained photomicrographs are shown. Even at the 10-fold-higher initial plating density, Ads-KD cells failed to form multinucleated osteoclasts, suggestive of a fusion defect.