Skip to main content
. 2014 Oct 2;9(10):e109257. doi: 10.1371/journal.pone.0109257

Figure 1. Effect of melatonin on light-induced DCFH2-DA oxidation.

Figure 1

A) Fluorescence spectrum (λexc = 485 nm, λem = 500–700 nm) of DCFH2-DA (100 µM) plus MEL (1 mM) under light (0–40 min). B) Fluorescence of several concentrations of DCFH2-DA plus MEL (1 mM) and light 60 minutes. ***p<0.001 vs no treat. C) Fluorescence of DCFH2-DA plus MEL (1 mM) during 5, 20 or 40 minutes under light or dark conditions. ***p<0.001 vs Darkness. D) Absorbance Spectrum (350–700 nm) of DCFH2-DA (100 µM) or MEL (1 mM) alone or mixed under 30 minutes of dark or light conditions. E) Absorbance measurement at 505 nm of MEL (1 mM), DCFH2-DA (100 µM), alone or mixed under 30 minutes of light or dark conditions. ***p<0.001 vs Darkness.