Table 2. List of 7 primer pairs used to differentiate 92 serotypes of S. pneumonia.

Reaction No.	Forward Primer (5′-3′)	Reverse Primer (5′-3′)	Accumulative number of differentiated serotypes	Simulated Melt curves shown in
1	GCAGTTTGTTGGACTGAC	TGGTACATAGGCATCACG	41	Dataset S1
2	ATCGCTTGGGCTTTTGCG	ATAGCCGCATCAATCACG	58	Dataset S2
3	TGGGATGCTTTCTGTGTG	CGCAAGCAGCTAAAAGCA	75	Dataset S3
4	ACCCTAGCTCAAGAAGTC	ACGATGACGAGCGACTTT	84	Dataset S4
5	CCTCCATATATGCAACAGGC	CCTGCCTGCAAGTCTTGA	87	Dataset S5
6	TCGGAGCCAATGGGTTGA	GTTAGCGGCTTGAGTTTG	90	Dataset S6
7	CAGAGGATGCTCTCGTCA	GGTAGTGGATCGGGATTG	92	Dataset S7