Figure 3. Testing the effects of homology on CapFlank.
Non-homologous amplicon libraries with or without M13 adaptors added (Figure S1) representing a potato blight PiRXLRc, giant squid mtDNA ND4, pigeon mtDNA COI, and grape chlorplast rbcL sequences were captured with blight panel A or pigeon panel B 200 bp baits. The capture libraries were then analysed by qPCR for the 250 bp amplicon products for each of the 4 amplicons. Captured libraries without M13 adaptors are shown in blue and with M13 adaptors added shown in red and demonstrate an increase in enrichment with M13 adaptor addition.