Figure 4. NaHS, PAG and GSH pretreatments differentially regulated seedling growth, (h)GSH content, and (h)GSH/(h)GSSG(h) ratio.

Corresponding phenotypes were photographed after 200 µM CdCl₂ treatment for 72 h, with or without 100 µM NaHS, 2 mM PAG, 1 mM GSH, individual or combination pretreatments for 6 h (A). Scale bar, 2 cm. Contents of (h)GSH (B), and the ratio of (h)GSH/(h)GSSG(h) (C) in root tissues were also analyzed after 200 µM CdCl₂ treatment for 12 h, with or without 100 µM NaHS, 2 mM PAG, 1 mM GSH, individual or combination pretreatment for 6 h. Values are means ± SD of three independent experiments with three replicates for each. Bars denoted by the same letter did not differ significantly at P<0.05 according to Duncan's multiple range test.