Figure 5. NaHS and GSH pretreatments alleviated Cd-induced ROS production, but blocked by PAG.

LSCM results (A). Seedlings were pretreated with or without 100 µM NaHS, 2 mM PAG, 1 mM GSH, individual or combination for 6 h, and then exposed to 200 µM CdCl₂ for another 6 h. After various treatments, the roots were respectively stained with H₂DCFDA, then washed thoroughly to removal extra dye and immediately photographed by LSCM. Scale bar, 0.5 mm. The relative DCF fluorescence intensity in the corresponding roots (B).