Figure 1. MTT (A) and tryphan blue (B) assay for cell viability.

HPMCs were incubated for 72 hr with 5.6 mM glucose (NG), NG+ mannitol (94.4 mM, NG+M), high glucose (100 mM, HG), or HG+0.1 µM, 1 µM, or 10 µM simvastatin (HG + statin). Cell viability was maintained at up to 1 µM simvastatin, but was decreased by 20% at 10 µM. *; p<0.05 vs. other groups.