Figure 10.

Summary of sites of double-strand cleavage of hairpin DNAs 12 and 13. Orange bases indicate randomized region of the original hairpin DNA library. Arrows of the same shape and color indicate paired cleavages. Black arrows correspond to coupled double-strand cleavage events, whereas red arrows indicate noncoupled double-strand cleavage events, resulting from two independent single-strand cleavages on opposite strands. Nucleotide colored in red indicates a primary site of double-strand DNA cleavage for a coupled double-strand cleavage event.