Fluorescence emission spectra resulting
from treatment of 16-nt
hairpin DNA-Cf15 with Fe(II)·BLM A5 in
the presence or absence of 64-nt hairpin DNAs. The reaction mixture
contained 0.72 μM Fe(II)·BLM A5, 0.72 μM
hairpin DNA-Cf15, and 0.72 μM hairpin DNA 2, 7, 12, or 13 in 10 mM Na
cacodylate buffer solution, pH 7.0, containing 100 mM NaCl. The fluorescence
emission spectra were obtained following excitation at 310 nm and
25 °C. Also shown is the fluoresence emission spectrum of the
16-nt hairpin DNA-Cf15 treated with BLM A5 in
the absence of any 64-nt hairpin DNA under the same conditions (“no
competitor”).