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. 2014 Sep 4;136(39):13715–13726. doi: 10.1021/ja505733u

Figure 8.

Figure 8

Analysis of bleomycin-induced double-strand cleavage of hairpin DNA 12. (A) Double-strand cleavage of [3′-32P]-end-labeled (lane 2) and [5′-32P]-end-labeled (lane 3) 64-nt hairpin DNA 12 by Fe(II)·bleomycin A5. Lane 1, [3′-32P]-end-labeled DNA alone; lane 2, 1.5 μM Fe(II)·BLM A5; lane 3, 1.5 μM Fe(II)·BLM A5; lane 4, [5′-32P]-end-labeled DNA alone. (B) Sequencing gel analysis of Fe(II)·bleomycin-induced sites of double-strand cleavage of [5′-32P]-end-labeled (lanes 1–7) and [3′-32P]-end-labeled (lanes 8–14) hairpin DNA 12. Each lane (except lanes 1 and 14) corresponds to a numbered cleavage band, shown in (A). Lane 1, Maxam–Gilbert G+A sequencing lane of [5′-32P]-end-labeled DNA 12; lane 2, band 3a; lane 3, band 3b; lane 4, band 3c; lane 5, band 3d; lane 6, band 3e; lane 7, band 3f; lane 8, band 2f; lane 9, band 2e; lane 10, band 2d; lane 11, band 2c; lane 12, band 2b; lane 13, band 2a; lane 14, Maxam–Gilbert G+A sequencing lane of [3′-32P]-end-labeled DNA 12.