Table 2. Binding of Fe(III)·BLM B₂ to Hairpin DNAs 2, 7, 12, and 13 at 10 mM NaCl concentration and 15 °C^a.

DNA	ka (× 105 M–1 s–1)	kd (× 10–2 s–1)	KA (ka/kd) (× 106 M–1)	KD (kd/ka) (× 10–9 M)	KA (× 106 M–1) (steady-state)
2b	1.8 ± 0.23	1.7 ± 0.22	10.5 ± 1.3	95 ± 12	9.4 ± 0.38; 0.20 ± 0.08
7	1.7 ± 0.12	1.0 ± 0.18	17.0 ± 2.5	58.8 ± 2.0	15.6 ± 0.28
12	3.3 ± 0.35	1.9 ± 0.28	17.3 ± 1.5	57.8 ± 1.9	17.5 ± 0.32
13	2.3 ± 0.24	0.75 ± 0.27	30.7 ± 1.8	32.5 ± 1.5	22.8 ± 0.25

^a10, 20, 40, 70, and 90 nM concentrations of Fe(III)·BLM B₂ were used to determine the kinetic rate constants. Error values given in the table were obtained during the fitting of data with Biacore T200 evaluation software. Based on reproducibility of results, the errors in the strong binding constants and kinetics constants are ±15%.

^bData from ref (12).