Table 1. Detailed compositional analysis of AIN-93G-based diets.

The level of dietary fat was approximately 6% of dry weight supplied as either soybean oil (control chow) or menhaden fish oil (LC-O3PUFA-enriched chow). Gas chromatography coupled with mass spectrometry demonstrated that the level of DHA and eicosapentaenoic acid (EPA) in the menhaden fish oil was 12.82-g and 6.91-g, respectively, per 100g of diet. The level of cholesterol was 0.582-g/100g. This amount was added to control diets to make the levels consistent with that of the fish oil diet.

Ingredient	AIN-93G Control diet (%)	AIN-93G Fish oil-enriched diet (%)
Casein	20	20
L-Cystine	0.3	0.3
Corn starch	39.7	39.7
Maltodextrin	13.2	13.2
Sucrose	10	10
Fiber	5	5
Vitamin mix	1	1
Mineral mix	3.5	3.5
Choline bitartrate	0.25	0.25
tBHQ	0.0014	0.0014
Soybean oil	7	0.77
Fish oil (DHA + EPA + cholesterol)	0	6.23
Cholesterol (added to match fish oil levels)	0.0121	0
Omega-3 Fatty Acids in Oils
DHA	<1 g/100 g soybean oil	12.82 g/100 g fish oil
EPA	<1 g/100 g soybean oil	6.91 g/100 g fish oil
omega-6:omega-3	7.5:1	1:3.7