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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Crit Rev Biochem Mol Biol. 2014 Jul 17;49(5):413–425. doi: 10.3109/10409238.2014.939608

Figure 4. Docking model of eIF5A–60S ribosome complex.

Figure 4

(A) EF-P–70S ribosome complex (PDB: 3HUW and 3HUX, (Blaha, Stanley, and Steitz, 2009)). 50S subunit is colored gray and 30S subunit is colored light cyan, EF-P is colored as described in Figure 3, and tRNAfMet in P site is colored green. (Right) Magnified view of EF-P and P-site tRNAfMet structures.

(B) Yeast eIF5A (PDB: 3ER0) was docked between the P and E sites of the yeast 60S ribosome (PDB: 3O30, (Ben-Shem et al., 2010)) by aligning with the structure of the EF-P–70S ribosome complex (panel A) and adjusting according to the results of hydroxyl radical cleavage data (Gutierrez et al., 2013). Positions of L1 stalk, P stalk and central protuberance (CP) on the 60S subunit are indicated; tRNAiMet is colored blue, and eIF5A is colored as described in Figure 3 with Lys51, the site of hypusine modification, colored black. (Right) Magnified view of docked eIF5A and P site tRNAiMet structures rotated 180°. A color version of the figure is available online.